Seminars: Wednesday 12:15-13:45 (every second week; Arbeitsraum Biochemie, Nonnenplan 4)
please send an e-mail to Prof. Börsch () if you intend to participate in the module and enrol to the module in Friedolin as well.
In conventional microscopy experiments fluorescence data are acquired from a large number of molecules. Since many molecules are interrogated simultaneously, their properties are averaged out. In contrast to this single-molecule experiments provide access to an incredible wealth of information. This course gives an introduction to single-molecule techniques and the analysis of the data. The topics covered in the course are listed below:
Introduction - 25 years of single molecule detection
Introduction - superresolution microscopy with single molecules
Single molecule fluorescence
Photophysics of single molecules
Photochemistry of single molecules
Photophysics and photochemistry of single molcules
Microscopy of single molecules, superresolutionmicroscopy
Single-molecule Förster resonance energy transfer (smFRET I)
Single-molecule anisotropy and smFRET
The ABEL trap
Fluorescence correlation spectrosopy I
Requirements to complete the module:
Oral exam at the end of the semeseter.
On this page you can find links to further resources (textbooks & websites) about topics covered in the course. Please note: Linked journal articles are only accessible from computers directly connected to the computer network of the Friedrich-Schiller-University Jena.