Module S3.02 - Single-molecule microscopy
Coordinator
Prof. Dr. Michael Börsch
Lecturers
- Single-Molecule Microscopy Group: Prof. Dr. Michael Börsch
- Insitute of Physiology II: Dr. Ralf Schmauder
Schedule
- Lectures: Tuesday 12:15-13:45 (Arbeitsraum Biochemie, Nonnenplan 4)
- Seminars: Wednesday 12:15-13:45 (every second week; Arbeitsraum Biochemie, Nonnenplan 4)
- please send an e-mail to Prof. Börsch () if you intend to participate in the module and enrol to the module in Friedolin as well.
Contents
In conventional microscopy experiments fluorescence data are acquired from a large number of molecules. Since many molecules are interrogated simultaneously, their
properties are averaged out. In contrast to this single-molecule experiments provide
access to an incredible wealth of information.
This course gives an introduction to single-molecule techniques and the analysis of the
data. The topics covered in the course are listed below:
| Lectures | Exercises | ||||
|
Tuesday |
Lecturer |
Content |
Wednesday 12.15-13.45 |
Lecturer | Content |
|
1 |
Börsch |
Introduction - 25 years of single molecule detection |
|||
|
2 |
Börsch |
Introduction - superresolution microscopy with single molecules |
|
Börsch, Schmauder |
Single molecule fluorescence |
|
3 |
Börsch |
Photophysics of single molecules |
|
||
|
4 |
Börsch |
Photochemistry of single molecules |
|
Schmauder |
Photophysics and photochemistry of single molcules |
|
5 |
Börsch |
Single-molecule imaging |
|
||
|
6 |
Börsch |
Single-molecule fluorescence |
Schmauder | Microscopy of single molecules, superresolutionmicroscopy | |
|
7 |
Börsch |
Single-molecule anisotropy |
|
||
|
8 |
Börsch |
Single-molecule Förster resonance energy transfer (smFRET I) |
|
Börsch |
Single-molecule anisotropy and smFRET |
|
9 |
Börsch |
smFRET II |
|||
|
10 |
Börsch |
smFRET III |
|
Börsch |
Single-molecule FRET |
|
11 |
Börsch |
Single-molecule tracking |
|||
|
12 |
Börsch | The ABEL trap | Schmauder | Single-molecule diffusion | |
|
13 |
Schmauder |
Fluorescence correlation spectrosopy I |
Requirements to complete the module:
- Oral exam at the end of the semeseter.
Recommended literature
On this page you can find links to further resources (textbooks & websites) about topics covered in the course.
Please note: Linked journal articles are only accessible from computers directly connected to the computer network of the Friedrich-Schiller-University Jena.
- W.E. Moerner
Single-Molecule Spectroscopy, Imaging, and Photocontrol: Foundations for Super-Resolution
Microscopy (Nobel Lecture)
Angew. Chem. Int. Ed. 54, 8067–8093 (2015) - E. Betzig
Single Molecules, Cells, and Super-Resolution Optics (Nobel Lecture)
Angew. Chem. Int. Ed. 54, 8034–8053 (2015) - S. W. Hell
Nanoscopy with Focused Light (Nobel Lecture)
Angew. Chem. Int. Ed. 54, 8054–8066 (2015) - W.P. Ambrose et al.
Single Molecule Fluorescence Spectroscopy at Ambient Temperature
Chem. Rev. 99, 2929-2956 (1999)