Module S3.01 - Biological microscopy
Coordinator
Prof. Dr. Christoph Biskup
Lecturers
- Biomolecular Photonics Group: Prof. Dr. Christoph Biskup, PD Dr. Christian Melle, Dr. Birgit Hoffmann
- Single-Molecule Microscopy Group: Prof. Dr. Michael Börsch
- BioImaging Group: Prof. Dr. Knut Holthoff
- Institute of Molecular Cell Biology: Prof. Dr. Carsten Hoffmann
- Institute of Physiology II: Dr. Ralf Schmauder
Components of the module
- Lectures: every Friday 14.15 - 15.45 (Lecture hall Humangenetik, if not stated otherwise) starting on Oct 20th.
- Exercise: Friday 16.00 - 17.30 (as announced in the table below, the location will be announced in the lectures)
- Recorded video material, accessible via the download page.
- Exercise questions for self study.
Requirements to complete this module
- Attendance to the lectures held in presence
- Self study of the virtual lectures and the recommended literature.
- Work on the exercises
- Successful participation in the written exam
Exam
- Date: 16.02.2024
- Time: 14.15 p.m.
- Location: Lecture hall Humangenetik
Contents
| Week | Lecturer | Content | Tasks & Links |
|
Week 1 |
Biskup |
Introduction
|
See the download page for a copy of the lecture slides |
|
Week 2 Meeting point: |
Biskup Hoffmann Kache |
Techniques in fluorescence microscopy I
|
Please, read the review article and watch the videos available on the download page.
|
| Week 3 03.11.2023 |
Biskup |
Fluorophores I
|
See the download page for a copy of the lecture slides and additional material. |
| Week 4 10.11.2023 |
Biskup |
Fluorophores II
|
Please, watch the videos on GFP available on the download page before the lecture. See the recommended literature tab for links to additional resources See the download page for a copy of the lecture slides and additional material. |
|
Week 5 |
Biskup |
Techniques in fluorescence microscopy II
|
Please, watch the videos available on the download page before the lecture. See the download page for lecture videos, lecture slides and additional material. |
|
Week 6 Meeting point: |
Biskup |
Förster Resonance Energy Transfer (FRET) |
See the download page for lecture videos, lecture slides and additional material. Watch the videos before the practical course. |
|
Week 7 |
Schmauder |
Illuminating molecular processes
|
Please, look at the videos available on the download page before the meeting. |
|
Week 8 |
Börsch |
Single molecule measurements
|
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Week 9 |
Börsch |
Superresolution techniques
|
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Week 10 |
Holthoff |
Fluorescent indicators
|
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Week 11 |
Holthoff |
Manipulation of cells and tissues with optical tools
|
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Week 12 |
Holthoff |
Application I - Measurements in slices and living animals |
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Week 13 |
Hoffmann |
Applications II
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| Week 14 02.02.2024 |
Biskup |
Applications III - Receptor channels
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Week 15 |
Biskup |
Q & A
|
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16.02.2024 |
Biskup |
Exam |
Requirements to complete the module:
- Evaluation of experiments performed during the course.
- Written exam at the end of the semeseter.