| Week |
Lecturer |
Content |
|
Unit 1
|
Biskup |
Introduction
- Aims of the course
- Tissue processing for light microscopy: Video created by the University of Adelaide
- Cell culture techniques: Slides used in the zoom meeting: pdf
- Review of basic optics: Slides used in the zoom meeting: pdf
|
|
Unit 2
|
Biskup
Hoffmann
Kache |
Techniques in fluorescence microscopy
- Introductory lecture: Slides
- Introductory reading: Please, read the paper of J. Lichtman, J.A. Conchello:
Fluorescence microscopy. Nat. Methods 2, 910-919 (2005).
- Practical course: Epifluorescence microscopy: Video
- Practical course: Confocal laser scanning microscopy (CLSM): Video
|
Unit 3
|
Biskup |
Fluorophores
- Organic fluorophores for biological imaging: Slides used in the zoom meeting: pdf
- Labelling techniques: Slides used in the zoom meeting: pdf
- Introductory reading to labelling techniques: B.N.G. Giepmans, S.R. Adams, M.H. Ellisman, R.Y.Tsien: The fluorescent toolbox for accessing protein location and function. Science 312, 217-224 (2006)
|
Unit 4
|
|
Genetically encoded fluorescence proteins
- Introductory video: Interview with professor Lars Thelander about the discovery of GFP, its heterologous expression and its application in current research: Link
- R.Y. Tsien: Video of the Nobel lecture: Video, Lecture slides, Lecture notes (Note: The first 9 minutes of the video might be difficult for you to understand. Do not get frustrated by this. Keep on watching.)
- Publications and links to the videos of the Nobel lectures of the other Nobel prize laureates can be found in the recommended literature tab.
- Genetically encoded fluorescence proteins - Introduction: Slides
- Genetically encoded fluorescence proteins - Applications: Slides
|
Unit 5
|
Biskup |
Fluorescence lifetime measurements (FLIM)
- Introductory lecture: Slides
- Practical course: Fluorescence lifetime imaging (FLIM): Video
|
|
Unit 6
|
Biskup |
Förster Resonance Energy Transfer (FRET)
- Introduction to FRET: Slides
- Principles & methods used to measure FRET
- acceptor photobleaching
- sensitized emission
- donor fluorescenece lifetime
|
|
Unit 7
|
Schmauder |
Illuminating molecular processes
- Introduction to Fluorescence Correlation Spectroscopy (FCS) and related techniques: Video (Link for Download, link to the stream will follow soon), Slides
|
|
Unit 8
|
Börsch |
Single molecule measurements
- Single photon counting, Single molecule measurements setups, ABEL trap, single molecule FRET: Slides
|
|
Unit 9
|
Börsch |
Superresolution techniques
|
|
Unit 10
|
Holthoff |
Fluorescent indicators
- Organic indicators for pH, ion concentrations and membrane potential: Slides
|
|
Unit 11
|
Holthoff |
Manipulation of cells and tissues with optical tools
- Caged compounds, photochromic switches, optogenetics: Slides
|
|
Unit 12
|
Hoffmann |
Applications I - GPCRs
- Measurement of receptor activation: Slides
|
|
Unit 13
|
Holthoff |
Application II - Measurements in slices and living animals
|
Unit 14
|
Biskup |
Applications III - Receptor channels
- Measurement of ligand binding and receptor activation: Slides
|
|
Unit 15
|
Biskup |
Summary
- Review of the topics of the module
- Preparation for the exam
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