Dr. Maren Godmann, Prof. Dr. Torsten Heinzel, Dr. Christian Kosan, Dr. Julia von Maltzahn, PD Dr. Jörg Müller,
Practical course: at the beginning of the lecture free period of the winter semester
Please contact Dr. Babovsky if you intend to participate.
This module is part of the M.Sc. Molecular Medicine programme (MolMed S.16), but is offered also to students of the M.Sc. Medical photonics programme.
It gives an introduction into molecular biological methods used in medical research and diagnostics, including:
Genetic manipulation of microbial and eukaryotic cells: purification of DNA, use of DNA-modifying enzymes, gene cloning, PCR, mutagenesis, transformation, transfection, transduction
Genome editing: TALEN, ZFN, CRISPR
DNA sequence analysis: Introduction of different systems, applications
Quantification of gene expression: RT-qPCR, gene reporters systems, array techniques
Protein analysis: immunological techniques, protein synthesis, covalent protein modification
In addition, methods for analysis of protein-DNA-interaction (EMSA, DNase I footprinting; interference footprinting, two-hybrid-systems, FRET) and bacterial and eukaryotic rDNA gene expression systems will be introduced. Further, up-to-date techniques in molecular biological medical diagnostics (flow cytometry, gendiagostic approaches, single cell characterization, molecular imaging and cytogenetics) will be explained and practiced.