Module S3.01 - Biological microscopy
Coordinator
Prof. Dr. Christoph Biskup
Lecturers
- Biomolecular Photonics Group: Prof. Dr. Christoph Biskup, PD Dr. Christian Melle, Dr. Birgit Hoffmann
- Single-Molecule Microscopy Group: Prof. Dr. Michael Börsch
- BioImaging Group: Prof. Dr. Knut Holthoff
- Institute of Molecular Cell Biology: Prof. Dr. Carsten Hoffmann
- Institute of Physiology II: Dr. Ralf Schmauder
Components of the module
- Lectures: every Friday 14.15 - 15.45 (Lecture hall Humangenetik, if not stated otherwise) starting on Oct 21st.
- Exercise: Friday 16.00 - 17.30 (as announced in the table below, the location will be announced in the lectures)
- Recorded video material, accessible via the download page.
- Exercise questions for self study.
Requirements to complete this module
- Attendance to the lectures held in presence
- Self study of the virtual lectures and the recommended literature.
- Work on the exercises
- Successful participation in the written exam
Exam
- Date: 17.02.2023
- Time: 14.15 p.m.
- Location: Lecture hall Humangenetik
Contents
Week | Lecturer | Content | Tasks & Links |
Week 1 |
Biskup |
Introduction
|
See the download page for a copy of the lecture slides |
Week 2 |
Biskup Hoffmann Kache |
Techniques in fluorescence microscopy
|
Please, read the review article and watch the videos available on the download page.
|
Week 3 04.11.2022 |
Biskup |
Fluorophores
|
See the download page for a copy of the lecture slides and additional material. |
Week 4 11.11.2022 |
Biskup |
Fluorophores
Techniques in fluorescence microscopy
|
Please, watch the videos on GFP available on the download page before the meeting. See the recommended literature tab for links to additional resources See the download page for a copy of the lecture slides and additional material. |
Week 5 |
Biskup |
Techniques in fluorescence microscopy (Cont.)
|
Please, look at the videos available on the download page before the meeting. See the download page for lecture videos, lecture slides and additional material. |
Week 6 Meeting point: |
Biskup |
Förster Resonance Energy Transfer (FRET) |
See the download page for lecture videos, lecture slides and additional material. |
Week 7 |
Schmauder |
Illuminating molecular processes
|
Please, look at the videos available on the download page before the meeting. |
Week 8 |
Börsch |
Single molecule measurements
|
|
Week 9 |
Börsch |
Superresolution techniques
|
|
Week 10 |
Holthoff |
Fluorescent indicators
|
|
Week 11 |
Holthoff |
Manipulation of cells and tissues with optical tools
|
|
Week 12 |
Holthoff |
Application I - Measurements in slices and living animals |
|
Week 13 |
Hoffmann |
Applications I
|
|
Week 14 03.02.2023 |
Biskup |
Applications III - Receptor channels
|
|
Week 15 |
Biskup |
Q & A
|
|
17.02.2023 |
Biskup |
Exam |
Requirements to complete the module:
- Evaluation of experiments performed during the course.
- Written exam at the end of the semeseter.