Module S3.03 - Electron microscopy
Coordinator
PD Dr. Martin Westermann
Lecturers
PD Dr. Martin Westermann, Dr. Sandor Nietzsche, Prof. Dr. Klaus Jandt, PD Dr. Jörg Bossert, PD Dr. Stephanie Höppener, Prof. Dr. Ulrich Sigmar Schubert
Schedule
- Lectures: every Thursday 08.15 - 09.45 (SR Ziegelmühlenweg 1), starting on Oct 18th.
- Exercise: Thursday 10.00 - 11.30 (SR Ziegelmühlenweg 1) according to the table below
Contents
This module provides an introduction into the fundamentals of electron microscopy. The following topics will be covered in the course:
| Week | Lecturers | Lecture 08.15-09.45 |
Exercises 10.00-11.30 |
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|
1 |
Westermann / Nietzsche |
Introduction to Electron Microscopy History, principles & theories Download:slides |
Demonstration of electron microscopes | |
|
2 |
Westermann / Nietzsche |
Instrumentation I Transmission electron microscopy Download: slides |
TEM TEM setup, TEM handling, TEM imaging |
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3 |
Westermann / Nietzsche |
Instrumentation II Scanning electron microscopy |
SEM SEM specimen preparation, SEM signals, SEM imaging |
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4 |
Westermann / Nietzsche |
Instrumentation III Energy dispersive X-ray spectroscopy in SEM |
EDX EDX of different samples |
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5 |
Westermann / Nietzsche |
Electron microscopy of biologic samples I Processing of tissue specimens |
Imaging of biologic samples I Embedding, Ultramicroscopy, Section imaging |
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6 |
Westermann / Nietzsche |
Electron microscopy of biologic samples II Cryotechniques, freeze-fracture electron microscopy Download: slides |
Imaging of biologic samples II Rapid freezing, freeze fracturing, replica imaging |
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7 |
Westermann / Nietzsche |
Electron microscopy of biologic samples III Tomograpy, cristallography, immunolabelling, negative staining Download: slides |
Imaging of biologic samples II Imaging of labeled replicas, negative stainuing of TMV, ferritin |
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8 |
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9 |
Bossert |
Electronmicroscopy of biomaterials |
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10 |
Bossert |
Electronmicroscopy of biomaterials |
Electronmicroscopy of biomaterials |
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11 |
Höppener / Schubert |
Electronmicroscopy of polymers I |
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12 |
Höppener / Schubert |
Electronmicroscopy of polymers II |
Electronmicroscopy of polymers | |
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13 |
Höppener / Schubert |
Electronmicroscopy of polymers III |
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14 |
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15 |
Westermann / Nietzsche | Questions and answers |
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