Module S3.02 - Single-molecule microscopy
Coordinator
Prof. Dr. Michael Börsch
Lecturers
- Single-Molecule Microscopy Group: Prof. Dr. Michael Börsch
- Insitute of Physiology II: Dr. Ralf Schmauder
Schedule
- Lectures: every Tuesday 12.15 - 13.45 (Arbeitsraum Biochemie), starting on Oct 16th.
- Exercise: every second Wednesday 12.15 - 13.45 (Arbeitsraum Biochemie)
Contents
In conventional microscopy experiments fluorescence data are acquired from a large number of molecules. Since many molecules are interrogated simultaneously, their
properties are averaged out. In contrast to this single-molecule experiments provide
access to an incredible wealth of information.
This course gives an introduction to single-molecule techniques and the analysis of the
data. The topics covered in the course are listed below:
| Lectures | Exercises | ||||
|
Tuesday |
Lecturer |
Content |
Wednesday 12.15-13.45 |
Lecturer | Content |
|
1 |
Börsch |
Introduction - 25 years of single molecule detection Download: slides |
|||
|
2 |
Börsch |
Introduction - superresolution microscopy with single molecules Download: slides |
|
Börsch, Schmauder |
Single molecule fluorescence |
|
3 |
Börsch |
Photophysics of single molecules Download: slides |
|
||
|
4 |
Börsch |
Photochemistry of single molecules |
|
Schmauder |
Photophysics and photochemistry of single molcules |
|
5 |
Börsch |
Single-molecule imaging Download: slides |
|
||
|
6 |
Börsch |
Single-molecule fluorescence Download: slides |
Schmauder | Microscopy of single molecules, superresolutionmicroscopy | |
|
7 |
Börsch |
Single-molecule anisotropy Download: slides |
|
||
|
8 |
Börsch |
Single-molecule Förster resonance energy transfer (smFRET I) |
|
Börsch |
Single-molecule anisotropy and smFRET |
|
9 |
Börsch |
smFRET II |
|||
|
10 |
Börsch |
smFRET III |
|
Börsch |
Single-molecule FRET |
|
11 |
Börsch |
Single-molecule tracking |
|||
|
12 |
Börsch | The ABEL trap | Schmauder | Single-molecule diffusion | |
|
13 |
Schmauder |
Fluorescence correlation spectrosopy I |
|||
|
14 |
Schmauder | Fluorescence correlation spectrosopy II | Schmauder | Fluorescence correlation spectrosopy | |
|
15 |
Börsch, Schmauder | Questions and answers |
Requirements to complete the module:
- Oral exam at the end of the semeseter.
Recommended literature
On this page you can find links to further resources (textbooks & websites) about topics covered in the course.
Please note: Linked journal articles are only accessible from computers directly connected to the computer network of the Friedrich-Schiller-University Jena.
- W.E. Moerner
Single-Molecule Spectroscopy, Imaging, and Photocontrol: Foundations for Super-Resolution
Microscopy (Nobel Lecture)
Angew. Chem. Int. Ed. 54, 8067–8093 (2015) - E. Betzig
Single Molecules, Cells, and Super-Resolution Optics (Nobel Lecture)
Angew. Chem. Int. Ed. 54, 8034–8053 (2015) - S. W. Hell
Nanoscopy with Focused Light (Nobel Lecture)
Angew. Chem. Int. Ed. 54, 8054–8066 (2015) - W.P. Ambrose et al.
Single Molecule Fluorescence Spectroscopy at Ambient Temperature
Chem. Rev. 99, 2929-2956 (1999)