The aim of this course is to present modern methods of Mass Spectrometry Imaging (MSI) for the analysis of complex tissue sections. These techniques like MALDI (Matric assisted laser desorption and ionization) Imaging or Desorption ionization electrospray imaging (DESI) are especially used for the analysis of tumor tissues. The potential as well as the limitations of these label-free spectrometric techniques towards a more differentiated medical diagnosis will be discussed.
Why (MALDI) imaging?
Mass spectrometers, types and technical principles
Other MS Imaging techniquws (SIMS etc)
How MALDI Imaging works (example, from patient to image)
Working with differant material (Kryo, FFPE)
MALDI Imaging technical aspects
On-slide digestion and matrix application
MALDI Imaging for the analysis of organs and complex tissue
Ambient MS imaging
DESI analysis of tissues ex vivo, intelligent knife in-vivo
Looking for biomarkers
Identification of biomarkers (MS/MS or other)
Correlation to other imaging techniques (Raman, MRI etc.)
Image analysis by different bioinformatic tools (segmentation, machine learning)
Exercises will be organized in blocks every four weeks: