Module S3.11 - Mass Spectrometry Imaging
Coordinator/Lecturer: apl. Prof. Dr. Ferdinand von Eggeling
Schedule:
- Lectures: every Wednesday 08.15 - 09.45 (Stoystr. 3, office Eggeling)
starting on Oct 18th. - Exercise: every fourth Wednesday 14.15 - 17.45 (Stoystr. 3, office Eggeling)
Contents:
The aim of this course is to present modern methods of Mass Spectrometry Imaging (MSI)
for the analysis of complex tissue sections. These techniques like MALDI (Matric assisted
laser desorption and ionization) Imaging or Desorption ionization electrospray imaging
(DESI) are especially used for the analysis of tumor tissues.
The potential as well as the limitations of these label-free spectrometric techniques towards
a more differentiated medical diagnosis will be discussed.
| Week | Content |
|
1 |
Why (MALDI) imaging? |
|
2 |
Mass spectrometers, types and technical principles |
|
3 |
Other MS Imaging techniquws (SIMS etc) |
|
4 |
How MALDI Imaging works (example, from patient to image) |
|
5 |
Working with differant material (Kryo, FFPE) |
|
6 |
MALDI Imaging technical aspects |
|
7 |
On-slide digestion and matrix application |
|
8 |
MALDI Imaging for the analysis of organs and complex tissue |
|
9 |
3D MALDI |
|
10 |
Ambient MS imaging |
|
11 |
DESI analysis of tissues ex vivo, intelligent knife in-vivo |
|
12 |
Looking for biomarkers |
|
13 |
Identification of biomarkers (MS/MS or other) |
|
14 |
Correlation to other imaging techniques (Raman, MRI etc.) |
|
15 |
Image analysis by different bioinformatic tools (segmentation, machine learning) |
Exercises will be organized in blocks every four weeks:
| 1. | Tissue Preparation |
| 2. | On slide digestion and Matrix application |
| 3. | MALDI Imaging |
| 4. | Software analysis |
Requirements to complete the module:
- written exam at the end of the semeseter
Exam dates:
- to be announced in the lecture