Module S3.03 - Electron microscopy
Coordinator: PD Dr. Martin Westermann
Lecturers:
PD Dr. Martin Westermann, Dr. Sandor Nietzsche, Prof. Dr. Klaus Jandt, PD Dr. Jörg Bossert, PD Dr. Stephanie Höppener, Prof. Dr. Ulrich Sigmar Schubert
Schedule:
- Lectures: every Thursday 08.15 - 09.45 (SR Ziegelmühlenweg 1), starting on Oct 19th.
- Exercise: Thursday 10.00 - 11.30 (SR Ziegelmühlenweg 1) according to the table below
Contents:
This module provides an introduction into the fundamentals of electron microscopy. The following topics will be covered in the course.
| Week | Lecturers | Lecture 08.15-09.45 |
Exercises 10.00-11.30 |
|
1 |
Westermann / Nietzsche |
Introduction to Electron Microscopy History, principles & theories Download: slides |
Demonstration of electron microscopes |
|
2 |
Westermann / Nietzsche |
Instrumentation I Transmission electron microscopy Download: slides |
TEM TEM setup, TEM handling, TEM imaging |
|
3 |
Westermann / Nietzsche |
Instrumentation II Scanning electron microscopy |
SEM SEM specimen preparation, SEM signals, SEM imaging |
|
4 |
Westermann / Nietzsche |
Instrumentation III Energy dispersive X-ray spectroscopy in SEM |
EDX EDX of different samples |
|
5 |
Westermann / Nietzsche |
Electron microscopy of biologic samples I Processing of tissue specimens Download: slides |
Imaging of biologic samples I Embedding, Ultramicroscopy, Section imaging |
|
6 |
Westermann / Nietzsche |
Electron microscopy of biologic samples II Cryotechniques, freeze-fracture electron microscopy Download: slides |
Imaging of biologic samples II Rapid freezing, freeze fracturing, replica imaging |
|
7 |
Westermann / Nietzsche |
Electron microscopy of biologic samples III Tomograpy, cristallography, immunolabelling, negative staining |
Imaging of biologic samples II Imaging of labeled replicas, negative stainuing of TMV, ferritin |
|
8 |
|
|
|
|
9 |
Bossert |
Electronmicroscopy of biomaterials |
|
|
10 |
Bossert |
Electronmicroscopy of biomaterials |
Electronmicroscopy of biomaterials |
|
11 |
Höppener / Schubert |
Electronmicroscopy of polymers I |
|
|
12 |
Höppener / Schubert |
Electronmicroscopy of polymers II |
Electronmicroscopy of polymers |
|
13 |
Höppener / Schubert |
Electronmicroscopy of polymers III |
|
|
14 |
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|
15 |
Westermann / Nietzsche | Questions and answers |
|
Download additional material:
Requirements to complete the module
- Written exam at the end of the semeseter.
Exam dates
- to be announced in the lecture