Lab Team

Joachim Clement, Dr. rer. biol. hum. (Group Leader)

Abteilung Hämatologie und Internistische Onkologie
Klinik Innere Medizin II
Universitätsklinikum Jena
Phone.: +49 (3641) 9-325820
Fax: +49 (3641) 9-325822
email:

Lena Belfi, B.Sc., Masterstudent
Cornelia Jörke, MTA
Frauke Seeber, Doktorandin
Malika Sutter, B.Sc., Masterstudentin
Martin Taeger, Doktorand
Ekaterina Zheveliuk, M.Sc., Doktorandin, LG Stipendiatin

Research Focus

The entire nature of tumor cells and their communication with the microenvironment is complex. We are interested in the mechanism, which drive the dissemination of tumor cells from the primary tumor and allow the survival of these cells in the circulation and finally their evasion. An import role in these processes plays the bone morphogenetic protein signaling network. In collaboration with partners from academia and industry we develop new procedures to identify and isolate the entirety of circulating tumor cells from the peripheral blood. Novel photonic technologies and innovative nanomaterials contribute to a significant part to this approach.

Innovative nanomaterials in hematology and oncology

Magnetic metallic nanoparticles are widely used tools to isolate and label cells, e.g. circulating tumor cells. We are interested in understanding the cellular processes during the interaction of nanomaterials with human cells. In focus are overall cell vitality, intracellular transport mechanisms and inflammatory response. The life cycle of nanomaterials is studied in long-term cell culture models.

Identification and characterization of circulating tumor cells

The detection and identification of circulating tumor cells is challenging. The proportion of these cells is very low in the peripheral blood of tumor patients. The cell population is highly variable which requires an in-depth investigation on a single cell level. A further characterization on the molecular level is needed for future advances in cancer diagnosis and therapy.

Tumor biology of Bone Morphogenetic Proteins

Bone morphogenetic proteins are a subgroup of the Transforming Growth Factor beta superfamily. They are important regulators of embryogenesis, organogenesis and tumorigenesis. We could show that BMP-2 keeps cells in a “susceptible state” that can take various directions depending on further signals.

Publications (Selection)

2020

Rosenfeldt, S., Mickoleit, F., Jörke, C., Clement, J.H., Markert, S., Jérôme, V., Schwarzinger, S., Freitag, R., Schüler, D., Uebe, R., Schenk, A.S. 2020. Towards standardized purification and regeneration of bacterial magnetic nanoparticles for future in vivo applications. Acta Biomaterialia: https://doi.org/10.1016/j.actbio.2020.07.042

Dutz, S., Buske, N., Landers, J., Gräfe, C., Wende, H., Clement, J.H. 2020. Biocompatible magnetic fluids of Co-doped iron oxide nanoparticles with tunable magnetic properties. Nanomaterials: 10: 1019; doi.org/10.3390/nano10061019

2019

Mihajlović, J., Diehl, L.M.A., Hochhaus, A., Clement J.H. 2019. Inhibition of bone morphogenetic protein signaling reduces viability, growth and migratory potential of non-small cell lung carcinoma cells. J. Cancer Res. Clin. Oncol. 145: 2675-2687.

Alcala, N., Leblay, N., Gabriel, A., Mangiante, L., Hervas Marin, D., Giffon, T., Sertier, A.S., Ferrari, a., Derks, J., Ghantous, A., Delhomme, T.M., Chabrier, A., Cuenin, C., Abedi-Ardekani, B., Boland, A., Caux, C., Altmueller, A., Durand, G., Voegele, C., Boyault, S., Lemaitre, N., Lorimier, P., Toffart, A.C., Mc Leer, A., Solterman, A., Clement, J.H., Saenger, J., Field, J.K., Brevet, M., Blanc-Fournier, C., Galateau-Salle, F., Le Stang, N., Damiola, F., Russell, P., Wright, G., Roz, L., Lacomme, S., Vignaug, J.M., Hofman, V., Hofman, P., Brustugun, O.T., Lund-Iversen, M., Thomas de Montpreville, V., Muscarella, L.A., Graziano, P., Volante, M., Papotti, M.G., Brcic, L., Popper, H., Deleuze, J.F., Herceg, Z., Viari, A., Nuernberg, P., McKay, J.D., Sandoval, J., Hernandez-Vargas, H., Brambilla, E., Speel, E.J., Girard, N., Lantuejoul, S., Foll, M., Fernandez-Cuesta, L. 2019. Integrative and comparative analyses of multi-omics data identify clinically relevant molecular groups of pulmonary carcinoids and unveil the existence of supra-atypical carcinoids. Nature Commun. 10: 3407; doi.org/10.1038/s41467-019-11276-9

Gräfe, C., von der Lühe, M., Weidner, A., Globig, P., Clement, J.H., Dutz, S., Schacher, F.H. 2019. Protein Corona Formation and its Constitutional Changes on Magnetic Nanoparticles in Serum featuring a Polydehydroalanine Coating: Effects of Charge and Incubation Conditions. Nanotechnology 30(26):265707. doi: 10.1088/1361-6528/ab0ed0

2018

Müller, E.K., Gräfe, C., Wiekhorst, F., Bergemann, C., Weidner, A., Dutz, S., Clement, J.H. 2018. Magnetic nanoparticles interact and pass an in vitro co-culture blood-placenta model. Nanomaterials 8: 108; doi:10.3390/nano8020108

George, J., Walter, V., Peifer, M., Alexandrov, L.B., Seidel, D., Leenders, F., Maas, L., Müller, C., Dahmen, I., Delhomme, T.M., Ardin, M., Leblay, N., Byrnes, G., Sun, R., De Reynies, A., McLeer-Florin, A., Bosco, G., Malchers, F., Menon, R., Altmüller, J., Becker, C., Nürnberg, P., Achter, V., Lang, U., Schneider, P.M., Bogus, M., Soloway, M.G., Wilkerson, M.D., Cun, Y., McKay, J.D., Moro-Sibilot, D., Brambilla, C.G., Lantuejoul, S., Lemaitre, N., Soltermann, A., Weder, W., Tischler, V., Brustugun, O.T., Lund-Iversen, M., Helland, Å., Solberg, S., Ansén, S., Wright, G., Solomon, B., Roz, L., Pastorino, U., Petersen, I., Clement, J.H., Sänger, J., Wolf, J., Vingron, M., Zander, T., Perner, S., Travis, W.D., Haas, S.A., Olivier, M., Foll, M., Büttner, R., Hayes, D.N., Brambilla, E., Fernandez-Cuesta, L., Thomas, R.K. 2018. Integrative genomic profiling of large-cell neuroendocrine carcinomas reveals distinct subtypes of high-grade neuroendocrine lung tumors. Nat Commun. 9: 1048.

Complete list of publications here.

Theses and Dissertations

We encourage applications for Bachelor’s and Master’s theses as well as doctoral theses in the fields of medicine (experimental) and the life sciences. For more information, please contact Dr. Joachim Clement ().

Funding

Deutsche Forschungsgemeinschaft
BMBF
Dr. Rainald Stromeyer-Stiftung