Lab Team

Joachim Clement, Dr. rer. biol. hum. (Group Leader)

Abteilung Hämatologie und Internistische Onkologie
Klinik Innere Medizin II
Universitätsklinikum Jena
Phone.: +49 (3641) 9-325820
Fax: +49 (3641) 9-325822
email:

Lennart Gresing, B.Sc., Masterstudent
Cornelia Jörke, MTA
Frauke Seeber, Doktorandin
Malika Sutter, B.Sc., Masterstudentin
Martin Taeger, Doktorand
Ekaterina Zheveliuk, M.Sc., Doktorandin, LG Stipendiatin

Research Focus

The entire nature of tumor cells and their communication with the microenvironment is complex. We are interested in the mechanism, which drive the dissemination of tumor cells from the primary tumor and allow the survival of these cells in the circulation and finally their evasion. An import role in these processes plays the bone morphogenetic protein signaling network. In collaboration with partners from academia and industry we develop new procedures to identify and isolate the entirety of circulating tumor cells from the peripheral blood. Novel photonic technologies and innovative nanomaterials contribute to a significant part to this approach.

Innovative nanomaterials in hematology and oncology

Magnetic metallic nanoparticles are widely used tools to isolate and label cells, e.g. circulating tumor cells. We are interested in understanding the cellular processes during the interaction of nanomaterials with human cells. In focus are overall cell vitality, intracellular transport mechanisms and inflammatory response. The life cycle of nanomaterials is studied in long-term cell culture models.

Identification and characterization of circulating tumor cells

The detection and identification of circulating tumor cells is challenging. The proportion of these cells is very low in the peripheral blood of tumor patients. The cell population is highly variable which requires an in-depth investigation on a single cell level. A further characterization on the molecular level is needed for future advances in cancer diagnosis and therapy.

Tumor biology of Bone Morphogenetic Proteins

Bone morphogenetic proteins are a subgroup of the Transforming Growth Factor beta superfamily. They are important regulators of embryogenesis, organogenesis and tumorigenesis. We could show that BMP-2 keeps cells in a “susceptible state” that can take various directions depending on further signals.

Publications (Selection)

2019

Mihajlović, J., Diehl, L.M.A., Hochhaus, A., Clement J.H. 2019. Inhibition of bone morphogenetic protein signaling reduces viability, growth and migratory potential of non-small cell lung carcinoma cells. J. Cancer Res. Clin. Oncol. 145: 2675-2687.

Alcala, N., Leblay, N., Gabriel, A., Mangiante, L., Hervas Marin, D., Giffon, T., Sertier, A.S., Ferrari, a., Derks, J., Ghantous, A., Delhomme, T.M., Chabrier, A., Cuenin, C., Abedi-Ardekani, B., Boland, A., Caux, C., Altmueller, A., Durand, G., Voegele, C., Boyault, S., Lemaitre, N., Lorimier, P., Toffart, A.C., Mc Leer, A., Solterman, A., Clement, J.H., Saenger, J., Field, J.K., Brevet, M., Blanc-Fournier, C., Galateau-Salle, F., Le Stang, N., Damiola, F., Russell, P., Wright, G., Roz, L., Lacomme, S., Vignaug, J.M., Hofman, V., Hofman, P., Brustugun, O.T., Lund-Iversen, M., Thomas de Montpreville, V., Muscarella, L.A., Graziano, P., Volante, M., Papotti, M.G., Brcic, L., Popper, H., Deleuze, J.F., Herceg, Z., Viari, A., Nuernberg, P., McKay, J.D., Sandoval, J., Hernandez-Vargas, H., Brambilla, E., Speel, E.J., Girard, N., Lantuejoul, S., Foll, M., Fernandez-Cuesta, L. 2019. Integrative and comparative analyses of multi-omics data identify clinically relevant molecular groups of pulmonary carcinoids and unveil the existence of supra-atypical carcinoids. Nature Commun. 10: 3407; doi.org/10.1038/s41467-019-11276-9

Gräfe, C., von der Lühe, M., Weidner, A., Globig, P., Clement, J.H., Dutz, S., Schacher, F.H. 2019. Protein Corona Formation and its Constitutional Changes on Magnetic Nanoparticles in Serum featuring a Polydehydroalanine Coating: Effects of Charge and Incubation Conditions. Nanotechnology 30(26):265707. doi: 10.1088/1361-6528/ab0ed0

2018

Müller, E.K., Gräfe, C., Wiekhorst, F., Bergemann, C., Weidner, A., Dutz, S., Clement, J.H. 2018. Magnetic nanoparticles interact and pass an in vitro co-culture blood-placenta model. Nanomaterials 8: 108; doi:10.3390/nano8020108

George, J., Walter, V., Peifer, M., Alexandrov, L.B., Seidel, D., Leenders, F., Maas, L., Müller, C., Dahmen, I., Delhomme, T.M., Ardin, M., Leblay, N., Byrnes, G., Sun, R., De Reynies, A., McLeer-Florin, A., Bosco, G., Malchers, F., Menon, R., Altmüller, J., Becker, C., Nürnberg, P., Achter, V., Lang, U., Schneider, P.M., Bogus, M., Soloway, M.G., Wilkerson, M.D., Cun, Y., McKay, J.D., Moro-Sibilot, D., Brambilla, C.G., Lantuejoul, S., Lemaitre, N., Soltermann, A., Weder, W., Tischler, V., Brustugun, O.T., Lund-Iversen, M., Helland, Å., Solberg, S., Ansén, S., Wright, G., Solomon, B., Roz, L., Pastorino, U., Petersen, I., Clement, J.H., Sänger, J., Wolf, J., Vingron, M., Zander, T., Perner, S., Travis, W.D., Haas, S.A., Olivier, M., Foll, M., Büttner, R., Hayes, D.N., Brambilla, E., Fernandez-Cuesta, L., Thomas, R,K. 2018. Integrative genomic profiling of large-cell neuroendocrine carcinomas reveals distinct subtypes of high-grade neuroendocrine lung tumors. Nat Commun. 9: 1048.

2017

Dutz, S., Wojahn, S., Gräfe, C., Weidner, A., Clement, J.H. 2017. Influence of sterilization and preservation procedures on the integrity of protein coated magnetic nanoparticles. Nanomaterials 7(12): 453

Allenstein, U., Wisotzki, E.I., Gräfe, C., Clement, J.H., Liu, Y., Schroers, J., Mayr, S.G. 2017. Binary FePd nanostructures fabricated through glancing angle deposition (GLAD) for bioapplications. Materials & Design 131: 366-374

Schlenk, F., Werner, S., Rabel, M., Jakobs, F., Bergemann, C., Clement, J.H.,Fischer, D. 2017. Comprehensive Analysis of the in vitro and ex vivo hemocompatibility of surface engineered iron oxide nanoparticles for biomedical applications.  Arch Toxicology 19(10): 3271-3286

Billing, M., Gräfe, C., Saal, A., Biehl, P., Clement, J.H., Dutz, S., Weidner, A., Schacher, F.H. 2017. Zwitterionic Iron Oxide (Fe2O3) Nanoparticles Based on P(2VP-grad-AA) Copolymers. Macromolecular Rapid Communications 38(4): 1600637

Göhring, A., Reuter, S., Clement, J.H., Cheng, X., Theobald, J., Wölfl, S., Mrowka, R. 2017. Human microRNA-299-3p decreases invasive behavior of cancer cells by downregulation of Oct4 expression and causes apoptosis. PLosONE 12(4): e0174912

Fedorov, L., Tian, Q., Gromov, P., Clement, J.H., Wang, Y., Riemann, M., Weih, F., Sun, X.-X., Dai, M. 2017. RHEB1 insufficiency in aged male mice is associated with stress-induced seizures. GeroScience: 39: 557-570

2016

Freitag, I., Beleites, C., Dochow, S., Clement, J.H., Krafft, C., Popp, J. 2016. Recognition of Tumor Cells by Immune-SERS-Markers in a Microfluidic Chip at Continuous Flow. The Analyst 141: 5986-5989

Gräfe, C., Slabu, I., Wiekhorst, F., Bergemann, C., von Eggeling, F., Hochhaus, A., Trahms, L., Clement, J.H. 2016. A model system for quantitative study of iron oxide nanoparticle passage through an endothelial cell layer. Phys Med Biol 61: 3986-4000

Tian, Q., Smart, J., Clement, J.H., Wang, Y., Derkatch, A., Schubert, H., Danilchik, M.V., Marks, D.L., Fedorov, L.M. 2016. Rheb expression in embryonic and postnatal mouse. Histochem Cell Biol 145: 561-572

Gräfe, C., Weidner, A., von der Lühe, M., Bergemann, C., Schacher, F.H., Clement, J.H., Dutz, S. 2016. Intentional formation of a protein corona on nanoparticles: Serumconcentration affects protein corona mass, surface charge, and nanoparticle–cell interaction. J Biochem Cell Biol 75: 196-202

2015

Zhou, M., Liebert, T., Müller, R., Dellith, A., Gräfe, C., Clement, J.H., Heinze, T. 2015. Magnetic biocomposites for remote melting. Biomacromolecules 16: 2308-2315

Weidner, A., Gräfe, C., von der Lühe, M., Remmer, H., Clement, J.H., Eberbeck, D., Ludwig, F., Müller, R., Schacher, F.H., Dutz, S. 2015. Preparation of core-shell hybrid materials by producing a protein corona around magnetic nanoparticles.  Nanoscale Research Letters: DOI 10.1186/s11671-015-0992-2

Steinbach, C., Steinbrücker, C., Pollok, S., Walther, K., Clement, J.H., Chen, Y., Petersen, I., Cialla-May, D., Weber, K., Popp, J. 2015. KRAS mutation screening by chip-based DNA hybridization – a further step towards personalized oncology, The Analyst 140: 2747-2754

Freitag, I., Matthäus, C., Csaki, A., Clement, J.H., Weber, K., Krafft, C., Popp, J. 2015. Differentiation of MCF-7 tumor cells from leukocytes and fibroblast cells by EpCAM functionalized multicore SERS labels. J. Biomed. Optics 20: 55002

Theumer, A., Gräfe, C., Bähring, F., Bergemann, C., Hochhaus, A., Clement, J.H. 2015. Superparamagnetic iron oxide nanoparticles exert different cytotoxic effects on cells grown in monolayer cell culture versus as multicellular spheroids. J. Magn. Magn. Mater. 380: 27-33

Fernandez-Cuesta, L., Sun, R., Menon, R., George, J., Lorenz, S., Meza-Zepeda, L.A., Peifer, M., Plenker, D., Heuckman, J.M., Leenders, F., Zander, T., Dahmen, I., Koker, M., Schöttle, J., Ullrich, R.T., Altmüller, J., Becker, C., Nürnberg, P., Seidel, H., Böhm, D., Göke, F., Ansén, Russell, P.A., Wright, G.M., Wainer, Z., Solomon, B., Petersen, I., Clement, J.H., Sänger, J., Brustugun, O.T., Helland, A., Solberg, S., Lund-Iversen, M., Buettner, R., Wolf, J., Brambilla, E., Vingron, M., Perner, S., Haas, S.A., Thomas, R.K. 2015. Identification of novel fusion genes in lung cancer using breakpoint assembly of transcriptome sequencing data. Genome Biology 16: 7

Von der Lühe, M., Günther, U., Weidner, A., Gräfe, C., Clement, J.H., Dutz, S., Schacher, F. 2015. SPION@Polydehydroalanine Hybrid Particles, RSC Advances 5: 31920 – 31929

Complete list of publications here.

Theses and Dissertations

We encourage applications for Bachelor’s and Master’s theses as well as doctoral theses in the fields of medicine (experimental) and the life sciences. For more information, please contact Dr. Joachim Clement ().

Funding

Deutsche Forschungsgemeinschaft
BMBF
Dr. Rainald Stromeyer-Stiftung