Air pollution poses a growing global health challenge, with fine particulate matter (PM) contributing substantially to the burden of respiratory diseases and premature death. PM exposure is closely linked to chronic respiratory diseases and increases susceptibility to respiratory infections by compromising epithelial integrity and weakening innate immunity. Influenza A viruses (IAVs) are among the most relevant respiratory pathogens and can cause severe and sometimes life-threatening lung diseases, particularly in vulnerable individuals. Recent findings suggest that PM may further exacerbate the severity of viral infections. However, the molecular mechanisms influencing pathogen load, host cell homeostasis, and immune regulation remain poorly understood. Building on an established in vitro infection model for IAVs, current studies are investigating PM-mediated modulation of pathogen-host interactions, focusing on virus-induced signalling pathways, inflammatory responses, and cell death mechanisms.

During the summer school, participants will contribute to ongoing experiments with cellular infection models, both with and without exposure to environmental pollutants. The training includes sterile cell culture techniques under BSL-2 conditions, as well as the practical application of methods such as plaque titration and Western blot analysis.