Below you will find some review articles, which will give you an introduction into fluorescence microscopy and techniques for measuring fluorescence lifetimes.
For legal reasons we are not allowed to provide you with the pdfs of the articles but only with the links to the respective publications. However, inside the compueter network of the Friedrich-Schiller-University Jena all publications are accessible.
K. Suhling, L.M. Hirvonen, J.A. Levitt, P.H. Chung, C. Tregidgo, A. Le Marois, D.A. Rusakov, K. Zheng, S. Ameer-Beg, S. Poland, S. Coelho, R. Henderson, N. Krstajic: Fluorescence lifetime imaging (FLIM): Basic concepts and some recent developments. Medical Photonics 27, 3-40 (2015).
N. Boens, W. Qin, N. Basarić, J. Hofkens, M. Ameloot, J. Pouget, J.P. Lefèvre, B. Valeur, E. Gratton, M. vandeVen, N.D. Silva Jr, Y. Engelborghs, K. Willaert, A. Sillen, G. Rumbles, D. Phillips, A.J. Visser, A. van Hoek, J.R. Lakowicz, H. Malak, I. Gryczynski, A.G. Szabo, D.T. Krajcarski, N. Tamai N, A. Miura: Fluorescence lifetime standards for time and frequency domain fluorescence spectroscopy. Anal. Chem. 79, 2137-2149 (2007).
Fluorescence lifetime measurements of Coumarin 6:
U.S. Raikar, C.G. Renuka, Y.F Nadaf, B.G. Mulimani, A.M. Karguppikar, M.K. Soudagar: Solvent effects on the absorption and fluorescence spectra of coumarins 6 and 7 molecules: Determination of ground and excited state dipole moment. Spectrochim Acta A 65, 673-677 (2006).