Research Group Neuroglia
Methods and Techniques
Animal models: |
mice (wildtype and transgenic), postnatal and adult rats, postnatal and adult |
---|---|
Experimental Lesion Models: |
retinal ischemia (elevated IOP)glaucoma (episcleral vein cauterization)optic nerve axotomyoptic nerve crush
|
Intraocular delivery of substances: |
defined factors e.g., EPO |
Cell culture: |
Primary culture: retinal ganglion cells Müller glia microglia |
Cell lines: RGC-5 (Retinal ganglion cells) MIO-M1 (Müller glia) |
|
Cell isolation: |
FACS immunopanning |
Molecular biology: |
qPCR |
Protein biochemistry: |
Western blot |
Immunocyto- and histochemistry: |
multi-colour immunofluorescence |
Microscopy: |
confocal laser scanning microscopy (LSM) with multi-channel analysis and 3-D reconstruction |
In vivo analysis of visual function: |
virtual optomotor systemanalysis of intrinsic optical signals (IOS) |
Behavioural tests: |
visual water task |