Module S3.11 - Mass Spectrometry Imaging

Coordinator/Lecturer

apl. Prof. Dr. Ferdinand von Eggeling, Dr. Anna Mühlig, David Pertzborn

Schedule

Lectures: Monday 14:15-15:45 (Am Klinikum 1, Gebäude F2, 00.060)

please contact Prof. von Eggeling if you want to participate in this module -
and enrol to the module in Friedolin
Exercise on agreement (Am Klinikum 1, Gebäude F2, 00.060)

The aim of this course is to present modern methods of Mass Spectrometry Imaging (MSI)
for the analysis of complex tissue sections. These techniques like MALDI (Matric assisted
laser desorption and ionization) Imaging or Desorption ionization electrospray imaging
(DESI) are especially used for the analysis of tumor tissues.
The potential as well as the limitations of these label-free spectrometric techniques towards
a more differentiated medical diagnosis will be discussed.

Week	Content
1	Why (MALDI) imaging? Additional information can be found in DOSIS: soon
2	Mass spectrometers, types and technical principles
3	Other MS Imaging techniques (SIMS etc)
4	How MALDI Imaging works (example, from patient to image)
5	Working with differant material (Kryo, FFPE)
6	MALDI Imaging technical aspects (spatial resolution)
7	MALDI Imaging technical aspects (mass resolution)
8	DESI analysis of tissues ex vivo, intelligent Knife in-vivo
9	Looking for of biomarker and Identification of Biomarker
10	Correlation to spectroscopic imaging techniques
11	Fancy imaging techniques
12	Multimodal and augmented reality
13	How to find literature and about scientific social media
14	Exam I
15	Exam II

Exercises will be organized in blocks every four weeks:

1.	Tissue Preparation
2.	On slide digestion and Matrix application
3.	MALDI Imaging
4.	Software analysis

Requirements to complete the module:

oral exam at the end of the semester

Module S3.11 - Mass Spectrometry Imaging

Coordinator/Lecturer

Schedule

Contents