Recommended literature and additional resources

On this page you can find links to further resources (textbooks & websites) about topics covered in the course. Publications, which you should have read before the respective lectures starts, are highlighted in red.
Please note: Linked pdfs are only accessible from computers directly connected to the computer network of the Friedrich-Schiller-University Jena.

Optics

Books

Eugene Hecht
Optics, 4th ed.
Addison-Wesley Longman, Amsterdam 2003.
B.E.A. Saleh, M.C. Teich
Fundamentals of Photonics, 2nd ed.
John Wiley & Sons, New York, Chichester, Brisbane, Toronto, Singapore 2007

Microscopy

Review articles

J. Lichtman, J.A. Conchello:
Fluorescence microscopy.
Nat. Methods 2, 910-919 (2005)

Books

James B. Pawley (Ed.):
Handbook of biological confocal microscopy, 3rd ed.
Springer, Singapore 2006.

World Wide Web
Tutorials of microscope companies (in alphabetical order):

Fluorescence

Books

J. R. Lakowicz
Principles of fluorescence spectroscopy. 3rd ed.
Springer, Singapore 2006.

Fluorescence lifetime imaging

Review articles

K. Suhling, P.W.M. French, D. Phillips:
Time resolved fluorescence microscopy.
Photochem. Photobiol. Sci. 4, 13-22 (2005)

Books

A. Periasamy, R.M. Clegg
FLIM Microscopy in biology and medicine.
CRC Press, Boca Raton, London, New York 2009.

Organic fluorophores and labelling techniques

Review articles on labelling techniques and green fluorescent proteins

Introduction into labelling techniques:

B.N.G. Giepmans, S.R. Adams, M.H. Ellisman, R.Y.Tsien:
The fluorescent toolbox for accessing protein location and function.
Science 312, 217-224 (2006)

Genetically encoded fluorescent proteins

Overview about GFPs

N.C. Shaner, P.A. Steinbach, R.Y. Tsien:
A guide to choosing fluorescent proteins.
Nat. Meth. 2, 905-909 (2005).

Discovery of GFP

O. Shimomura:
Video of the Nobel lecture: Video, Lecture slides, Lecture notes
O. Shimomura:
A short story of aequorin
Biol. Bull. 189, 1-5 (1995).
O. Shimomura:
Discovery of green fluorescent protein (GFP) (Nobel Lecture).
Angew. Chem. Int. Ed. 48, 5590-5602 (2009).

GFP as a genetic marker

M. Chalfie:
Video of the Nobel lecture: Video, Lecture slides, Lecture notes

Modificaton of GFP

R.Y. Tsien:
Video of the Nobel lecture: Video, Lecture slides, Lecture notes
R.Y. Tsien:
Nobel lecture: constructing and exploiting the fluorescent protein paintbox.
Integr. Biol. 2, 77-93 (2010)

Additional Information

See also the website of the Nobel Prize committee for additional information

Announcement of the 2008 Nobel Prize in Chemistry to Osamu Shimomura, Martin Chalfie and Roger Y. Tsien, presented by Professor Gunnar Öquist, Secretary General of the Royal Swedish Academy of Sciences, on October 8th, 2008: Link
Interview with professor Lars Thelander about the discovery of GFP, its heterologous expression and its application in current research: Link
But see also B. Lieff Benderly: The man who wasn't there. Science-Website

FRET

Review articles

A. Kenworthy:
Imaging protein-protein interactions using fluorescence resonance energy transfer microscopy.
Methods 24, 289-296 (2001)
C. Biskup et. al.:
Multi-dimensional fluorescence lifetime and FRET measurements.
Microsc Res Tech. 70, 442-451 (2007)