Module S3.01 - Biological microscopy
Coordinator
Prof. Dr. Christoph Biskup
Lecturers
- Biomolecular Photonics Group: Prof. Dr. Christoph Biskup, PD Dr. Christian Melle, Dr. Birgit Hoffmann
- Single-Molecule Microscopy Group: Prof. Dr. Michael Börsch
- BioImaging Group: Prof. Dr. Knut Holthoff
- Institute of Molecular Cell Biology: Prof. Dr. Carsten Hoffmann
- Institute of Physiology II: Dr. Ralf Schmauder
Components of the module
- Virtual lectures: Please, follow the links below to view the lectures.
- Exercise questions for self study.
- Seminars: Online seminars/tutorials to discuss remaining questions. Please, follow the links below to participate in the meetings.
Requirements to complete this module
- Self study of the virtual lectures
- Self study of the recommended literature.
- Regular work on the exercises
- Successful participation in the written exam
Exam
- Date: t.b.a.
- Time: t.b.a.
- Location: Big lecture hall of the theoretical medical institutes
Contents
Week | Lecturer | Content | Download / Links |
Week 1 |
Biskup |
Zoom Meeting
|
Link to join the meeting: See the download page for a copy of the lecture slides |
Week 2 |
Biskup Hoffmann Kache |
Techniques in fluorescence microscopy
|
See the download page for lecture videos, lecture slides and additional material. |
Week 3
(16.11.-20.11.2020) |
Biskup |
Fluorophores
|
Material will be added at later times... |
Week 4 (23.11.-27.11.2020) |
Biskup |
Techniques in fluorescence microscopy (Cont.) |
See the download page for lecture videos, lecture slides and additional material. |
Week 5 |
Biskup |
Förster Resonance Energy Transfer (FRET) |
See the download page for lecture videos, lecture slides and additional material. |
Friday |
Biskup |
Zoom Meeting
|
Link to join the meeting: https://uni-jena-de.zoom.us/j/4128807326 The passcode is: 9PLFY3 |
Week 6 |
Schmauder |
Illuminating molecular processes
|
See the download page for lecture videos and lecture slides. |
Friday |
Schmauder |
Zoom Meeting
|
Link to join the meeting: |
Week 7 |
Börsch |
Single molecule measurements
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The lecture will be live via Zoom. You can find the link here: 70409639 |
Friday |
Börsch |
Zoom Meeting
|
Link to join the meeting: |
Week 8 |
Börsch |
Superresolution techniques
|
|
Friday |
Börsch |
Zoom Meeting
|
Link to join the meeting: |
Week 9 |
Holthoff |
Fluorescent indicators
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Friday |
Zoom Meeting
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Link to join the meeting: click here Meeting-ID: 953 6448 9924 The passcode is: j65RkS |
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Week 10 |
Holthoff |
Manipulation of cells and tissues with optical tools
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Friday |
Holthoff |
Zoom Meeting
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Link to join the meeting: click here Meeting-ID: 999 3228 0318 The passcode is: 9huB5S |
Week 11 |
Hoffmann |
Zoom lecture on Applications I |
Link to join the meeting: https://uni-jena-de.zoom.us/j/4128807326 The passcode is: 9PLFY3 |
Week 12 |
Holthoff, Kirmse |
Application II - Measurements in slices and living animals |
Link to join the meeting: |
Week 13
|
Biskup |
Applications III - Receptor channels |
|
Friday |
Biskup |
Zoom Meeting
|
Link to join the meeting: https://uni-jena-de.zoom.us/j/4128807326 The passcode is: 9PLFY3 |
Wednesday |
Biskup |
Exam |
|
Requirements to complete the module:
- Evaluation of experiments performed during the course.
- Written exam at the end of the semeseter.
Recommended literature
On this page you can find links to further resources (textbooks & websites) about topics covered in the course. Publications, which you should have read before the respective lectures starts, are highlighted in red.
Please note: Linked pdfs are only accessible from computers directly connected to the computer network of the Friedrich-Schiller-University Jena.
Link | |
Optics |
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Books B.E.A. Saleh, M.C. Teich |
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Microscopy |
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Review articles |
Link
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Books James B. Pawley (Ed.) Handbook of biological confocal microscopy, 3rd ed. Springer, Singapore 2006. |
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World Wide Web |
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Fluorescence |
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Books |
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Fluorescence lifetime imaging |
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Review articles |
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Books |
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Organic fluorophores and labelling techniques | |
Review articles on labelling techniques and green fluorescent proteins Introduction into labelling techniques: B.N.G. Giepmans, S.R. Adams, M.H. Ellisman, R.Y.Tsien: |
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Genetically encoded fluorescent proteins |
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Overview about GFPs: N.C. Shaner, P.A. Steinbach, R.Y. Tsien: Discovery of GFPs: O. Shimomura: Angew. Chem. Int. Ed. Engl. 48, 5590-5602 (2009) R.Y. Tsien: See also the website of the Nobel Prize committee But see also B. Lieff Benderly: The man who wasn't there. Science-Website |
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FRET |
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Review articles C. Biskup et. al.: |