Module S2.4 - Dyes and labels

Lecturers

Prof. Dr. Kalina Peneva ( )

Components of the module

Lectures: Thursdays, 14:15 - 15:45
The module is taught in presence, starting from April, 23rd
Students registered in Moodle will receive access to the lecture slides and are expected to review the content of the first two sections in preparation for the course start.
Course materials and further information will be provided via Moodle.
The module includes a limited self-study component, supported by reading material and follow-up work related to the lecture topics.
Special session (May 21st):
On May 21st, the lecture will take place as a joint session with Prof. Dr. Carsten Hoffmann in the context of the L³ theme week “What is Life?”. In this session, we will compare fluorescent proteins and synthetic dyes as complementary strategies to visualize biological systems, highlighting their design principles, applications, and combined use in modern fluorescence microscopy.

Course content

This module introduces the principles, design, synthesis, properties, and applications of dyes and labels used in modern fluorescence-based imaging and sensing. It covers the relationship between molecular structure and photophysical behaviour, strategies to tune chromophores, and their use in microscopy and biomedical contexts.

Topics include:

fundamentals of fluorescence and chromophore design
important dye classes and their characteristic properties
synthetic approaches toward functional fluorophores
tuning of absorption and emission properties
fluorophores for fluorescence microscopy and sensing

selected examples of labelling strategies and bio-related applications

Teaching format

The course combines lecture-based teaching with selected elements of guided self-study. Students are expected to follow the lecture content, review the provided material, and prepare selected topics independently.

Requirements to complete the module

regular attendance and active participation
independent preparation and follow-up of selected topics
completion of the written examination

Exam

Format: Written exam
Date: 02.07.2026

Schedule

23.04 – First lecture (in presence)
30.04 onward – Regular lectures every Thursday, 14:15 - 15:45
21.05 – Special joint session with Prof. Dr. Carsten Hoffmann (L³ theme week)
Further details and course materials will be communicated via Moodle